Recombinant Mouse ANGPT1 Protein (Ser20-Leu261 & Arg277-Phe498), C-6×His-tagged
Product Description
Cat
IMP-10192
Official Symbol
ANGPT1
Product Overview
Recombinant Mouse Angiopoietin-1 protein (Ser20-Leu261) and (Arg277-Phe498) with 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a secreted glycoprotein that belongs to the angiopoietin family. Members of this family play important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Mutations in this gene are associated with hereditary angioedema.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Ser20
Form
Lyophilized from a 0.2 μm filtered solution in Tris-Citrate and NaCl with Trehalose and with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 56 kDa
SDS-PAGE: 59-75 kDa, reducing conditions
Protein length
Ser20-Leu261 & Arg277-Phe498
Bio-activity
Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-50 ng/mL in the presence of 5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked Homooligomer
Storage
12 months from date of receipt, ≤ -20 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, ≤ -20 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in water.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit serum deprivation induced apoptosis in HUVEC human umbilical vein endothelial cells. The ED50 for this effect is 10-50 ng/mL in the presence of 5 μg/mL of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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