Recombinant Mouse ANGPTL4 Protein (Gln24-Ser410), C-6×His-tagged
Product Description
Cat
IMP-10197
Official Symbol
ANGPTL4
Product Overview
Recombinant Mouse Angiopoietin-like 4 protein (Gln24-Ser410) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a glycosylated, secreted protein containing a C-terminal fibrinogen domain. The encoded protein is induced by peroxisome proliferation activators and functions as a serum hormone that regulates glucose homeostasis, lipid metabolism, and insulin sensitivity. This protein can also act as an apoptosis survival factor for vascular endothelial cells and can prevent metastasis by inhibiting vascular growth and tumor cell invasion. The C-terminal domain may be proteolytically-cleaved from the full-length secreted protein. Decreased expression of this gene has been associated with type 2 diabetes. Alternative splicing results in multiple transcript variants. This gene was previously referred to as ANGPTL2 but has been renamed ANGPTL4.
Expression System
CHO
Species
Mouse
Tag
C-6×His
Predicted N Terminal
No results obtained: Gln24 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Molecular Mass
Predicted Molecular Mass: 44.2 kDa
SDS-PAGE: 55-65 kDa, reducing conditions
Protein length
Gln24-Ser410
Bio-activity
Measured by its ability to inhibit lipoprotein lipase activity. The IC50 value under conditions in which Recombinant Human Lipoprotein Lipase/LPL and p-nitrophenyl butyrate are present in 0.1 M sodium phosphate, 0.15 M NaCl, 0.5% Triton X-100, pH 7.2, is approximately 0.1-1 μg/mL.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Oligomer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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