Recombinant Mouse BTN1A1 Protein (Ala27-Trp247), C-6×His-tagged
Product Description
Cat
IMP-10167
Official Symbol
BTN1A1
Product Overview
Recombinant Mouse BTN1A1/Butyrophilin protein (Ala27-Trp247) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
Butyrophilin is the major protein associated with fat droplets in the milk. It is a member of the immunoglobulin superfamily. It may have a cell surface receptor function. The human butyrophilin gene is localized in the major histocompatibility complex (MHC) class I region of 6p and may have arisen relatively recently in evolution by the shuffling of exons between 2 ancestral gene families.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Ala27
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 25 kDa
SDS-PAGE: 34-42 kDa, reducing conditions
Protein length
Ala27-Trp247
Bio-activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE with silver staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.3-1.5 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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