Recombinant Mouse BTN2A2 Protein (Gln30-Trp248), C-6×His-tagged
Product Description
Cat
IMP-10180
Official Symbol
BTN2A2
Product Overview
Recombinant Mouse BTN2A2/Butyrophilin 2A2 protein (Gln30-Trp248) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes, which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6, consisting of seven genes belonging to the expanding B7/butyrophilin-like group, a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid, fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
No results obtained. Gln30 inferred from enzymatic pyroglutamate treatment revealing Phe31
Form
Lyophilized from a 0.2 μm filtered solution in PBS and EDTA.
Molecular Mass
Predicted Molecular Mass: 26 kDa
SDS-PAGE: 38-45 kDa, reducing conditions
Protein length
Gln30-Trp248
Bio-activity
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 1-5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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