Recombinant Mouse Btnl4 Protein (Gln29-Trp250), C-6×His-tagged
Product Description
Cat
IMP-10174
Official Symbol
Btnl4
Product Overview
Recombinant Mouse BTNL4 protein (Gln29-Trp250) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
Predicted to enable signaling receptor binding activity. Predicted to be involved in T cell receptor signaling pathway and regulation of cytokine production. Predicted to be active in external side of plasma membrane. Orthologous to human BTNL3 (butyrophilin like 3) and BTNL8 (butyrophilin like 8).
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
No results obtained. Gln29 inferred from enzymatic pyroglutamate treatment revealing Glu30
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.8-4 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in PBS.
Bioactivity-ELISA 1
Measured by its ability to inhibit IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.8-4 μg/mL.
