Recombinant Mouse Cd14 Protein, C-His&C-hFc-tagged
Product Description
Cat
IMP-1893
Official Symbol
Cd14
Product Overview
Recombinant extracellular domain (Met 1-Pro 345) of mouse CD14 (NP_033971.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Description
The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. Cluster of differentiation 14 (CD14) is a member of the CD system. It takes its name from its inclusion in the CD molecule surface marker proteins. CD14 exists in two forms: a form anchored into the membrane or a soluble form. CD14 was found expressed in macrophages, neutrophil granulocyte and dendritic cells. The major function is to serve as a co-receptor (along with TLR4 and MD-2) for the bacterial lipopolysaccharide (LPS) and other pathogen-associated molecular patterns.
Expression System
HEK293
Species
Mouse
Tag
C-His&C-hFc
Predicted N Terminal
Ser 16
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse CD14/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 578 amino acids and has a predicted molecular mass of 63.5 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CD14/Fc monomer is approximately 85-95 kDa due to glycosylation.
Protein length
Met1-Pro345
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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