Recombinant Mouse CD200 Protein (Gln31-Gly232), C-hFc-tagged
Product Description
Cat
IMP-10255
Official Symbol
Cd200
Product Overview
Recombinant Mouse CD200 protein (Gln31-Gly 232) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc
Predicted N Terminal
No results obtained: Gln31 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 49 kDa (monomer)
SDS-PAGE: 65-75 kDa, reducing conditions
Protein length
Gln31-Gly232
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD200 Fc Chimera that produces 50% of the optimal binding response is appoximately 5-30 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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