Recombinant Mouse CD200 Protein (Gln31-Gly232), C-hFc-tagged
Product Description
Cat
IMP-10255
Official Symbol
Cd200
Product Overview
Recombinant Mouse CD200 protein (Gln31-Gly 232) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a type I membrane glycoprotein containing two extracellular immunoglobulin domains, a transmembrane and a cytoplasmic domain. This gene is expressed by various cell types, including B cells, a subset of T cells, thymocytes, endothelial cells, and neurons. The encoded protein plays an important role in immunosuppression and regulation of anti-tumor activity. Alternative splicing results in multiple transcript variants encoding different isoforms.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc
Predicted N Terminal
No results obtained: Gln31 predicted
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 49 kDa (monomer)
SDS-PAGE: 65-75 kDa, reducing conditions
Protein length
Gln31-Gly232
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD200 R1 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD200 Fc Chimera that produces 50% of the optimal binding response is appoximately 5-30 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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