Recombinant Mouse CD22 Protein (Ser22-Arg702), C-hFc-tagged

Product Description

Cat

IMP-10266

Official Symbol

Cd22

Product Overview

Recombinant Mouse Siglec-2/CD22 protein (Ser22-Arg702) with a Human IgG1 (Pro100-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc

Predicted N Terminal

Ser22

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 103 kDa (monomer)
SDS-PAGE: 140-150 kDa, reducing conditions

Protein length

Ser22-Arg702

Bio-activity

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. The ED50 for this effect is 0.08-0.4 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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