Recombinant Mouse CD226 Protein (Glu19-Pro254), C-hFc-tagged
Product Description
Cat
IMP-10275
Official Symbol
Cd226
Product Overview
Recombinant Mouse DNAM-1/CD226 Fc Chimera protein (Glu19-Pro254) with a Human IgG1 (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc
Predicted N Terminal
Glu19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 53.9 kDa
SDS-PAGE: 70-90 kDa, reducing conditions
Protein length
Glu19-Pro254
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 is coated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED50 of 0.3-1.8 μg/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 is coated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED50 of 0.3-1.8 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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