Recombinant Mouse CD226 Protein (Glu19-Pro254), C-hFc-tagged
Product Description
Cat
IMP-10275
Official Symbol
Cd226
Product Overview
Recombinant Mouse DNAM-1/CD226 Fc Chimera protein (Glu19-Pro254) with a Human IgG1 (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene encodes a glycoprotein expressed on the surface of NK cells, platelets, monocytes and a subset of T cells. It is a member of the Ig-superfamily containing 2 Ig-like domains of the V-set. The protein mediates cellular adhesion of platelets and megakaryocytic cells to vascular endothelial cells. The protein also plays a role in megakaryocytic cell maturation. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc
Predicted N Terminal
Glu19
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 53.9 kDa
SDS-PAGE: 70-90 kDa, reducing conditions
Protein length
Glu19-Pro254
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 is coated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED50 of 0.3-1.8 μg/mL.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in sterile PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Nectin-2/CD112 is coated at 1 μg/mL, Recombinant Mouse DNAM-1/CD226 Fc Chimera binds with a typical ED50 of 0.3-1.8 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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