Recombinant Mouse Cd27 protein, C-mFc Tag
Product Description
Cat
IMP-160
Official Symbol
Cd27
Product Overview
Recombinant Mouse Cd27 protein(AA Thr 21-Arg 182)(Accession # P41272-1) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.
Expression System
HEK293
Species
Mouse
Tag
C-mFc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 45.1 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Thr 21-Arg 182
Endotoxin
Less than 0.1 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Mouse CD27, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Mouse CD70, His Tag, Avi Tag with a linear range of 1-16 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Mouse CD27, Mouse IgG2a Fc Tag at 2 ug/mL (100 uL/well) can bind Biotinylated Human CD27 Ligand, Fc Tag with a linear range of 1-16 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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