Recombinant Mouse Cd274 protein, C-His Tag

Product Description

Cat

IMP-213

Official Symbol

Cd274

Product Overview

Recombinant Mouse Cd274 protein(AA Phe 19-Thr 238)(Accession # NP_068693) is expressed from human 293 cells (HEK293). This protein carries a polyhistidine tag at the C-terminus.

Expression System

HEK293

Species

Mouse

Tag

C-His Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.6 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Thr 238

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse PD-L1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-BLI 1

Loaded Mouse PD-1, Fc Tag on ProteinA Biosensor, can bind Mouse PD-L1, His Tag with an affinity constant of 1.5 uM as determined in BLI assay (ForteBio Octet Red96e).

Bioactivity-SPR 1

Captured Mouse PD-1, Fc Tag on Protein A Chip can bind Mouse PD-L1, His Tag with an affinity constant of 2.16 uM as determined in SPR assay (Biacore T200).

Bioactivity-SPR 2

Captured Mouse PD-L1, His Tag on CM5 Chip via Anti-His antibody can bind Mouse PD-1, Fc Tag with an affinity constant of 1.37 uM as determined in SPR assay (Biacore T200).

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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