Recombinant Mouse Cd274 protein, C-mFc Tag

Product Description

Cat

IMP-211

Official Symbol

Cd274

Product Overview

Recombinant Mouse Cd274 protein(AA Phe 19-Thr 238)(Accession # NP_068693) is expressed from human 293 cells (HEK293). This protein carries a mouse IgG2a Fc tag at the C-terminus.

Expression System

HEK293

Species

Mouse

Tag

C-mFc Tag

Form

Lyophilized from sterile PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 51.8 kDa. The protein migrates as 65-85 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Phe 19-Thr 238

Endotoxin

Less than 0.1 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Mouse B7-1, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin with a linear range of 0.16-2.5 ug/mL.

Bioactivity-ELISA 2

Immobilized Mouse PD-L1, Mouse IgG2a Fc Tag, low endotoxin at 5 ug/mL (100 uL/well) can bind Biotinylated Mouse PD-1, Fc,Avitag with a linear range of 0.313-5 ug/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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