Recombinant Mouse Cd276 Protein, C-His-tagged, Biotinylated
Product Description
Cat
IMP-1564
Official Symbol
Cd276
Product Overview
Recombinant mouse CD276 (NP_598744.1) (Met1-Phe244) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Description
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
Expression System
HEK293
Species
Mouse
Tag
C-His
Predicted N Terminal
Val 29
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse CD276 comprises 227 amino acids and has a predicted molecular mass of 24.9 kDa. The apparent molecular mass of the protein is approximately 40.9 kDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Phe244
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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