Recombinant Mouse Cd276 Protein, C-His-tagged, Biotinylated
Product Description
Cat
IMP-1564
Official Symbol
Cd276
Product Overview
Recombinant mouse CD276 (NP_598744.1) (Met1-Phe244) was expressed with a C-terminal polyhistidine tag. The purified protein was biotinylated in vitro.
Description
B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
Expression System
HEK293
Species
Mouse
Tag
C-His
Predicted N Terminal
Val 29
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse CD276 comprises 227 amino acids and has a predicted molecular mass of 24.9 kDa. The apparent molecular mass of the protein is approximately 40.9 kDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Phe244
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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