Recombinant Mouse CD28 Protein (Lys21-Lys149), C-hFc and 6×His-tagged

Product Description

Cat

IMP-10384

Official Symbol

Cd28

Product Overview

Recombinant Mouse CD28 Fc Chimera protein (Lys21-Lys149) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc and 6×His

Predicted N Terminal

Lys21

Form

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Molecular Mass

Predicted Molecular Mass: 52 kDa (monomer)
SDS-PAGE: 60 kDa, reducing conditions

Protein length

Lys21-Lys149

Bio-activity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.3-1.2 μg/mL when stimulated with 1 μg/mL recombinant human B7-1 Fc Chimera in the presence of PHA.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 400 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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