Recombinant Mouse CD300LB Protein (Ile18-Tyr157), C-mFc-tagged
Product Description
Cat
IMP-10380
Official Symbol
Cd300lb
Product Overview
Recombinant Mouse CD300b/LMIR5 protein (Ile18-Tyr157) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Ile18
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 43.2 kDa (monomer)
SDS-PAGE: 55-58 kDa, reducing conditions
Protein length
Ile18-Tyr157
Bio-activity
Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.3-1.8 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

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