Recombinant Mouse CD300LB Protein (Ile18-Tyr157), C-mFc-tagged

Product Description

Cat

IMP-10380

Official Symbol

Cd300lb

Product Overview

Recombinant Mouse CD300b/LMIR5 protein (Ile18-Tyr157) with a Mouse IgG2A (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

CD300LB is a nonclassical activating receptor of the immunoglobulin (Ig) superfamily expressed on myeloid cells.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-mFc

Predicted N Terminal

Ile18

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 43.2 kDa (monomer)
SDS-PAGE: 55-58 kDa, reducing conditions

Protein length

Ile18-Tyr157

Bio-activity

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. The ED50 for this effect is 0.3-1.8 μg/mL. Optimal dilutions should be determined by each laboratory for each application.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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