Recombinant Mouse CD33 Protein (Gln17-Glu240), C-mFc-tagged
Product Description
Cat
IMP-10378
Official Symbol
Cd33
Product Overview
Recombinant Mouse Siglec-3/CD33 protein (Gln17-Glu240) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Gln17 inferred from enymatic pyroglutamate treatment revealing Asp18
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 52 kDa
SDS-PAGE: 55-75 kDa, under reducing conditions
Protein length
Gln17-Glu240
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-H Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec-3 Fc Chimera that produces 50% of the optimal binding response is 5-20 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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