Recombinant Mouse CD33 Protein (Gln17-Glu240), C-mFc-tagged
Product Description
Cat
IMP-10378
Official Symbol
Cd33
Product Overview
Recombinant Mouse Siglec-3/CD33 protein (Gln17-Glu240) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Enables protein phosphatase binding activity and sialic acid binding activity. Involved in several processes, including negative regulation of cytokine production; negative regulation of monocyte activation; and positive regulation of protein tyrosine phosphatase activity. Located in several cellular components, including Golgi apparatus; external side of plasma membrane; and peroxisome.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Gln17 inferred from enymatic pyroglutamate treatment revealing Asp18
Form
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Molecular Mass
Predicted Molecular Mass: 52 kDa
SDS-PAGE: 55-75 kDa, under reducing conditions
Protein length
Gln17-Glu240
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Siglec-H Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse Siglec-3 Fc Chimera that produces 50% of the optimal binding response is 5-20 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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