Recombinant Mouse Cd3d&Cd3e Protein, C-Flag&C-His-tagged
Product Description
Cat
IMP-4233
Official Symbol
Cd3d&Cd3e
Product Overview
Recombinant extracellular domain (Met 1-Ala 105) of mouse CD3D (P04235) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met 1-Asp 108) of mouse CD3E (P22646) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
Description
The CD3 complex mediates signal transduction.
Expression System
HEK293
Species
Mouse
Tag
C-Flag&C-His
Predicted N Terminal
Phe 22&Asp 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of mouse CD3D/CD3E comprises 254 (126+128) amino acids and has a calculated molecular mass of 29.2 (14.3+14.9) KDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 20 and 22 KDa in SDS-PAGE under reducing conditions.
Protein length
Met1-Asp108
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 85 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info