Recombinant Mouse Cd3d&Cd3e Protein, No Tag

Product Description

Cat

IMP-4235

Official Symbol

Cd3d&Cd3e

Product Overview

Recombinant extracellular domain (Met1-Ala105) of mouse CD3D (P04235) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Asp108) of mouse CD3E (P22646) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.

Description

The CD3 complex mediates signal transduction.

Expression System

HEK293

Species

Mouse

Tag

No Tag

Predicted N Terminal

Phe 22&Asp 23

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of mouse CD3D/CD3E comprises 669 (334+335) amino acids and has a calculated molecular mass of 75.4 (37.5+37.9) kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 36-55 kDa in SDS-PAGE under reducing conditions.

Protein length

Met1-Ala105 and Met1-Asp108

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 90 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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