Product Overview
Recombinant extracellular domain (Met1-Ala105) of mouse CD3D (P04235) was fused with the C-terminal his-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Asp108) of mouse CD3E (P22646) was fused with the C-terminal flag-tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3D/CD3E heterodimer was purified.
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of mouse CD3D/CD3E comprises 669 (334+335) amino acids and has a calculated molecular mass of 75.4 (37.5+37.9) kDa. As a result of glycosylation, the apparent molecular mass of mouse CD3D/CD3E heterodimer is approximately 36-55 kDa in SDS-PAGE under reducing conditions.
