Recombinant Mouse Cd3e&Cd3g Protein, C-hFc&His&C-hFc-tagged

Product Description

Cat

IMP-4241

Official Symbol

Cd3e&Cd3g

Product Overview

Recombinant extracellular domain (Met1-Asp108) of mouse CD3E (NP_031674.1) was fused with the C-terminal polyhistidine tagged Fc region of human IgG1 at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Ser116) of mouse CD3G (NP_033980.1) was fused with the Fc region of human IgG1 at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD3E/CD3G heterodimer was purified.

Description

The CD3 complex mediates signal transduction.

Expression System

HEK293

Species

Mouse

Tag

C-hFc&His&C-hFc

Predicted N Terminal

Asp 23&Gln 23

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of mouse CD3E/CD3G comprises 671 (336+335) amino acids and has a calculated molecular mass of 75.4 (38.1+37.3) KDa.

Protein length

Met1-Asp108 and Met1-Ser116

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

95.1 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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