Recombinant Mouse Cd40 protein, C-His Tag

Product Description

Cat

IMP-379

Official Symbol

Cd40

Product Overview

Recombinant Mouse Cd40 protein (NP_035741.2) (Val24-Arg193) was expressed in Yeast, fused with a polyhistidine tag at the C-terminus.

Description

CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.

Expression System

Yeast

Species

Mouse

Tag

C-His Tag

Predicted N Terminal

Val 24

Form

Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Molecular Mass

The recombinant mouse CD40 consists of 180 amino acids and predicts a molecular mass of 20.3 kDa.

Protein length

Val24-Arg193

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

> 95 % as determined by SDS-PAGE.

Storage

Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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