IMP-4244

Cd40lg

Recombinant soluble form of mouse CD40LG (P27548) (Gly 115-Leu 260) was fused with the Fc region of human IgG1 at the N-terminus.

The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD154, also known as CD40 ligand or CD40L, is a member of the TNF superfamily. While CD154 was originally found on T cell surface, its expression has since been found on a wide variety of cells, including platelets, mast cells, macrophages and NK cells. CD154's ability is achieved through binding to the CD40 on antigen-presenting cells (APC). In the macrophage cells, the primary signal for activation is IFN-γ from Th1 type CD4 T cells. The secondary signal is CD40L on the T cell, which interacting with the CD40 molecules, helping increase the level of activation.

HEK293

Mouse

N-hFc

Glu

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

The recombinant mouse CD40LG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 406 amino acids and has a calculated molecular mass of 44.4 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.

Gly115-Leu260

< 1.0 EU/μg of the protein as determined by the LAL method

> 80 % as determined by SDS-PAGE

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Measured by its binding ability in a functional ELISA. Immobilized Mouse CD40 His at 2 μg/mL (100 μL/well) can bind Mouse CD40 Ligand hFc, the EC50 of Mouse CD40 Ligand hFc is 60-300 ng/mL.