Store at -20 to -80 centigrade. After reconstitution with 250 uL of sterilized water, store at -20 to -80 centigrade. Aliquot to avoid repeated freezing and thawing.
SDS-PAGE
1 μg/lane in 4-20% Tris-Glycine gel Stained with Coomassie Blue
Lane 1: non-reducing conditions
Lane 2: reducing conditions
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