Description
This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene.
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP alpha/CD172a Fc Chimera is coated at 2 μg/mL, Recombinant Mouse CD47 Fc Chimera binds with an apparent Kd <0.6 nM. Also measured by its ability to antagonize mouse red blood cell adhesion to immobilized Recombinant Mouse SIRP alpha/CD172a Fc Chimera. The ED50 for this effect is 2.0-8.0 μg/mL. Optimal dilutions should be determined by each laboratory for each application.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
