Recombinant Mouse CD52 Protein (Thr27-Ser47), C-mFc-tagged
Product Description
Cat
IMP-10331
Official Symbol
Cd52
Product Overview
Recombinant Mouse CD52 protein (Thr27-Ser47) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.
Description
Involved in positive regulation of cytosolic calcium ion concentration. Predicted to be located in extracellular region and plasma membrane. Predicted to be intrinsic component of plasma membrane. Predicted to be active in sperm midpiece.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Thr27
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 29 kDa
SDS-PAGE: 32-36 kDa & 38-42 kDa, under reducing conditions
Protein length
Thr27-Ser47
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 Fc Chimera Protein is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD52 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 1.5-7.5 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 Fc Chimera Protein is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD52 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 1.5-7.5 μg/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info