Recombinant Mouse CD52 Protein (Thr27-Ser47), C-mFc-tagged

Product Description

Cat

IMP-10331

Official Symbol

Cd52

Product Overview

Recombinant Mouse CD52 protein (Thr27-Ser47) with a Mouse IgG2a (Glu98-Lys330) Fc tag at C-terminus was expressed in Mouse myeloma cell line.

Description

Involved in positive regulation of cytosolic calcium ion concentration. Predicted to be located in extracellular region and plasma membrane. Predicted to be intrinsic component of plasma membrane. Predicted to be active in sperm midpiece.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-mFc

Predicted N Terminal

Thr27

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 29 kDa
SDS-PAGE: 32-36 kDa & 38-42 kDa, under reducing conditions

Protein length

Thr27-Ser47

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Human Siglec-10 Fc Chimera Protein is immobilized at 2.5 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD52 Fc Chimera that produces 50% of the optimal binding response is found to be approximately 1.5-7.5 μg/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 250 μg/mL in PBS.

SDS-PAGE

Bioactivity-ELISA 1

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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