Product Overview
Recombinant mouse CD59a (NP_001104530.1) (Met 1-Lys 95), without the pro peptide, was fused with the Fc region of human IgG1 at the C-terminus.
Description
Protectin, a complement regulatory protein, also known as CD59, or MIRL (membrane inhibitor of reactive lysis) is a small protein that inhibits the complement membrane attack complex by binding C5b678 and preventing C9 from binding and polymerizing. The amino-terminal 25 amino acids represented a typical signal peptide sequence and the carboxy-terminal hydrophobic amino acids were characteristic for phosphatidylinositol-anchored proteins. It was found that the CD59/Protectin antigen is a small protein sometimes associated with larger components (45 and 80 kD) in urine. CD59/Protectin antigen was released from the surface of transfected COS cells by phosphatidylinositol-specific phospholipase C, demonstrating that it is attached to the cell membrane by means of a glycolipid anchor; it is therefore likely to be absent from the surface of affected erythrocytes in the disease paroxysmal nocturnal hemoglobinuria.
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The secreted recombinant mouse CD59a/Fc is a disulfide-linked homodimer. The reduced monomer comprises 313 amino acids and has a calculated molecular mass of 35.4 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 42 kDa band in SDS-PAGE under reducing conditions.
