Recombinant Mouse CD80 Protein (Asp37-Lys245), C-6×His-tagged

Product Description

Cat

IMP-10313

Official Symbol

Cd80

Product Overview

Recombinant Mouse B7-1/CD80 protein (Asp37-Lys245) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Asp37

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 25 kDa
SDS-PAGE: 39-54 kDa, reducing conditions

Protein length

Asp37-Lys245

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CTLA-4 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse B7-1/CD80 that produces 50% of the optimal binding response is approximately 5-30 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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