Recombinant Mouse CD80 Protein (Asp37-Lys245), C-6×His-tagged

Product Description

Cat

IMP-10313

Official Symbol

Cd80

Product Overview

Recombinant Mouse B7-1/CD80 protein (Asp37-Lys245) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-6×His

Predicted N Terminal

Asp37

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 25 kDa
SDS-PAGE: 39-54 kDa, reducing conditions

Protein length

Asp37-Lys245

Bio-activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CTLA-4 Fc Chimera is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Mouse B7-1/CD80 that produces 50% of the optimal binding response is approximately 5-30 ng/mL.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 500 μg/mL in PBS.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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