Recombinant Mouse CD80 Protein (Asp37-Lys245), C-hFc and 6×His-tagged

Product Description

Cat

IMP-10310

Official Symbol

Cd80

Product Overview

Recombinant Mouse B7-1/CD80 protein (Asp37-Lys245) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc and 6×His

Predicted N Terminal

Asp37

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 51.3 kDa (monomer)
SDS-PAGE: 80-90 kDa, reducing conditions

Protein length

Asp37-Lys245

Bio-activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is 5-30 ng/mL in the presence of PHA.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile, deionized water.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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