Recombinant Mouse CD80 Protein (Asp37-Lys245), C-hFc and 6×His-tagged

Product Description

Cat

IMP-10310

Official Symbol

Cd80

Product Overview

Recombinant Mouse B7-1/CD80 protein (Asp37-Lys245) with a Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.

Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

Expression System

Mouse myeloma cell line

Species

Mouse

Tag

C-hFc and 6×His

Predicted N Terminal

Asp37

Form

Lyophilized from a 0.2 μm filtered solution in PBS.

Molecular Mass

Predicted Molecular Mass: 51.3 kDa (monomer)
SDS-PAGE: 80-90 kDa, reducing conditions

Protein length

Asp37-Lys245

Bio-activity

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. The ED50 for this effect is 5-30 ng/mL in the presence of PHA.

Endotoxin

<0.10 EU/μg of the protein by the LAL method.

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Notes

Disulfide-linked homodimer

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.

Reconstitution

Reconstitute at 100 μg/mL in sterile, deionized water.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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