Recombinant Mouse Cd80 protein, C-Fc Tag

Product Description

Cat

IMP-233

Official Symbol

Cd80

Product Overview

Recombinant Mouse Cd80 protein(AA Val 38-Lys 245)(Accession # Q00609-1) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.

Expression System

HEK293

Species

Mouse

Tag

C-Fc Tag

Form

Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.

Molecular Mass

This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 50.3 kDa. The protein migrates as 66-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Val 38-Lys 245

Endotoxin

Less than 1.0 EU per ug by the LAL method.

Purity

>95% as determined by SDS-PAGE.

Storage

For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse B7-1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1

Immobilized Mouse B7-1, Fc Tag at 2 ug/mL (100 uL/well) can bind Mouse CTLA-4, His Tag with a linear range of 0.05-0.8 ng/mL.

Bioactivity-ELISA 2

Immobilized Mouse B7-1, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse PD-L1, mouse IgG2a Fc tag, low endotoxin with a linear range of 0.16-2.5 ug/mL.

Bioactivity-ELISA 3

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Mouse B7-1, Fc Tag with a linear range of 0.6-10 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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