Recombinant Mouse Cd86 protein, C-Fc Tag
Product Description
Cat
IMP-231
Official Symbol
Cd86
Product Overview
Recombinant Mouse Cd86 protein(AA Val 24-Glu 245)(Accession # NP_062261.3) is expressed from human 293 cells (HEK293). This protein carries a human IgG1 Fc tag at the C-terminus.
Expression System
HEK293
Species
Mouse
Tag
C-Fc Tag
Form
Lyophilized from sterile Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass
This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 51.9 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length
Val 24-Glu 245
Endotoxin
Less than 1.0 EU per ug by the LAL method.
Purity
>95% as determined by SDS-PAGE.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower. Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE
SDS-PAGE

Mouse B7-2, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA 1
Bioactivity-ELISA

Immobilized Mouse B7-2, Fc Tag at 5 ug/mL (100 uL/well) can bind Mouse CTLA-4, His Tag with a linear range of 0.4-13 ng/mL.

Bioactivity-ELISA 2
Bioactivity-ELISA

Immobilized Biotinylated Mouse CD28, His,Avitag at 1 ug/mL (100 uL/well) on Streptavidin precoated (0.5 ug/well) plate, can bind Mouse B7-2, Fc Tag with a linear range of 2-39 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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