Recombinant Mouse Cd8a&Cd8b1 Protein, C-Flag&His-tagged
Product Description
Cat
IMP-1330
Official Symbol
Cd8a&Cd8b1
Product Overview
Recombinant extracellular domain (Met1-Tyr196) of mouse CD8A (NP_001074579.1) was fused with a flag tag at the C-terminus, constructed the plasmid 1; Recombinant extracellular domain (Met1-Thr175) of mouse CD8B (NP_033988.1) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the mouse CD8A&CD8B heterodimer was purified.
Description
CD8 exists as a disulfide-linked dimer of either α and β chain or two α chains. The crystal structure of the N-terminal 113 amino acids of human CD8 revealed homology to immunoglobulin (Ig) variable domains with nine β strands divided into two β sheets, one of four and the other of five strands.
Expression System
HEK293
Species
Mouse
Tag
C-Flag&His
Predicted N Terminal
Lys 28&Leu 22
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of mouse CD8A&CD8B comprises 405 (210+195) amino acids and has a calculated molecular mass of 45.9 (23.7+22.2) KDa.
Protein length
Met1-Tyr196; Met1-Thr175
Endotoxin
< 1.0 EU/μg protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info