Recombinant Mouse CEACAM5 Protein (Gln35-Glu947), C-6×His-tagged
Product Description
Cat
IMP-10245
Official Symbol
Ceacam5
Product Overview
Recombinant Mouse CEACAM-5/CD66e protein (Gln35-Glu947) with a C-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Gln35
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 103 kDa
SDS-PAGE: 115-135 kDa, under reducing conditions
Protein length
Gln35-Glu947
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CEACAM-5/CD66e His-tag is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 binds with an ED50 of 0.1-0.9 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CEACAM-5/CD66e His-tag is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 binds with an ED50 of 0.1-0.9 μg/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
Contact Info
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  • 1-631-559-9269
    1-516-512-3133
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