Recombinant Mouse CEACAM5 Protein (Gln35-Glu947), C-mFc-tagged
Product Description
Cat
IMP-10243
Official Symbol
Ceacam5
Product Overview
Recombinant Mouse CEACAM-5/CD66e protein (Gln35-Glu947) with a Mouse IgG2a (Glu98-Lys330) at C-terminus tag was expressed in Mouse myeloma cell line.
Description
This gene encodes a cell surface glycoprotein that represents the founding member of the carcinoembryonic antigen (CEA) family of proteins. The encoded protein is used as a clinical biomarker for gastrointestinal cancers and may promote tumor development through its role as a cell adhesion molecule. Additionally, the encoded protein may regulate differentiation, apoptosis, and cell polarity. This gene is present in a CEA family gene cluster on chromosome 19. Alternative splicing results in multiple transcript variants.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-mFc
Predicted N Terminal
Gln35
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 130 kDa
SDS-PAGE: 125-145 kDa and 185-195 kDa, under reducing conditions.
Protein length
Gln35-Glu947
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse CEACAM-5/CD66e Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 binds with an ED50 of 0.1-0.8 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 400 μg/mL in PBS.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Binding Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CEACAM-5/CD66e Fc Chimera is immobilized at 0.5 μg/mL (100 μL/well), Recombinant Mouse Galectin-4 binds with an ED50 of 0.1-0.8 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info