Recombinant Mouse CLEC9A Protein (Lys57-Ile264), N-mFc-tagged
Product Description
Cat
IMP-10221
Official Symbol
Clec9a
Product Overview
Recombinant Mouse CLEC9a protein (Lys57-Ile264) with a Mouse IgG2A (Glu98-Lys330) Fc tag at N-terminus was expressed in Chinese Hamster Ovary cell line.
Description
CLEC9A is a group V C-type lectin-like receptor (CTLR) that functions as an activation receptor and is expressed on myeloid lineage cells.
Expression System
CHO
Species
Mouse
Tag
N-mFc
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 50.8 kDa (monomer)
SDS-PAGE: 60-65 kDa, reducing conditions
Protein length
Lys57-Ile264
Bio-activity
Measured by flow cytometry for its ability to bind a ligand expressed in necrotic EL-4 mouse lymphoblast cells. When 100 ng of Recombinant Mouse CLEC9a Fc Chimera is added to 5 × 10^5 EL-4 cells, >50% of necrotic EL-4 cells bind to the protein.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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