Recombinant Mouse Ctla4 Protein (38-161aa), C-hIgG-His-Tagged
Product Description
Cat
IMP-9533
Official Symbol
Ctla4
Product Overview
Recombinant mouse Ctla-4, fused to hIgG-His-tag at Cterminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
Ctla-4, also known as cytotoxic T-lymphocyte protein 4 isoform 1, is a type 1 membrane protein and a memberof the immunoglobulin-superfamily. Besides, this protein contains an extra cellular domain, a transmembranedomain and a cytoplasmic tail. It is generally expressed with highest levels in lymphoid tissues. Also, it is similarto T-cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 and CD28 effectively regulate to T cell responses as opposing signals transmitted through tworelated cell-surface receptors.
Expression System
Baculovirus
Species
Mouse
Tag
C-hIgG-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
40.6 kDa (363 aa)
Protein length
38-161
AA Sequence
IQVTQPSVVLASSHGVASFPCEYSPSHNTDEVRVTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYFVGMGNGTQIYVIDPEPCPDSD<LEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKHHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.5 mg/mL (determined by Absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range ≤150 ng/mL.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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