Recombinant Mouse CTLA4 Protein (Ala37-Phe162), C-hFc and 6×His-tagged
Product Description
Cat
IMP-10229
Official Symbol
Ctla4
Product Overview
Recombinant Mouse CTLA-4 protein (Ala37-Phe162) with Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc and 6×His
Predicted N Terminal
Ala37
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 41 kDa (monomer)
SDS-PAGE: 55 kDa, reducing conditions
Protein length
Ala37-Phe162
Bio-activity
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80 Fc Chimera in the presence of PHA.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80 Fc Chimera in the presence of PHA.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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