Recombinant Mouse CTLA4 Protein (Ala37-Phe162), C-hFc and 6×His-tagged
Product Description
Cat
IMP-10229
Official Symbol
Ctla4
Product Overview
Recombinant Mouse CTLA-4 protein (Ala37-Phe162) with Human IgG1 (Pro100-Lys330) Fc tag and 6×His tag at C-terminus was expressed in Mouse myeloma cell line.
Description
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
C-hFc and 6×His
Predicted N Terminal
Ala37
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 41 kDa (monomer)
SDS-PAGE: 55 kDa, reducing conditions
Protein length
Ala37-Phe162
Bio-activity
Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80 Fc Chimera in the presence of PHA.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Notes
Disulfide-linked homodimer
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Bioactivity-ELISA 1
Bioactivity

Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.4 μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80 Fc Chimera in the presence of PHA.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

Online Inquiry
Contact Info