Recombinant Mouse CX3CL1 Protein (Met29-Lys102)
Product Description
Cat
IMP-10237
Official Symbol
Cx3cl1
Product Overview
Recombinant Mouse CX3CL1/Fractalkine protein (Met29-Lys102) without tag was expressed in E. coli.
Description
This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections.
Expression System
E. coli
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Met29
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 8.4 kDa
Protein length
Met29-Lys102
Bio-activity
Measured by its ability to antagonize Recombinant Mouse CX3CL1/Fractalkine aa 25-105 induced chemotaxis of BaF3 mouse pro-B cells transfected with mouse CX3CR1. The ED50 for this effect is 0.25-1.5 μg/mL in the presence of 50 ng/mL of Recombinant Mouse CX3CL1/Fractalkine aa 25-105. Optimal concentration depends on cell type as well as the application or research objective.
Endotoxin
<0.01 EU/μg of the protein by the LAL method.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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