Purified recombinant protein of Mouse cytochrome P450, family 2, subfamily e, polypeptide 1 (Cyp2e1), with C-terminal Myc/DDK tag, expressed in HEK293T cells.
Description
A cytochrome P450 monooxygenase involved in the metabolism of fatty acids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids. May be involved in the oxidative metabolism of xenobiotics.[UniProtKB/Swiss-Prot Function]
> 80% as determined by SDS-PAGE and Coomassie blue staining.
Storage
Store at -80 centigrade after receiving vials. Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
Concentration
> 50 μg/mL as determined by microplate BCA method.
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