Recombinant Mouse DKK1 Protein (Met1-Ser144), C-6×His-tagged
Product Description
Cat
IMP-9992
Official Symbol
DKK1
Product Overview
Recombinant Mouse Dkk-1 N-Terminal Fragment protein (Met1-Ser144) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP9 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines.
Expression System
CHO
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Ser30 & Thr32
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 13 kDa
SDS-PAGE: 13-22 kDa, reducing conditions
Protein length
Met1-Ser144
Bio-activity
Measured by its binding ability in a functional ELISA. When Recombinant Mouse Dkk-1 N-Terminal Fragment is coated at 1 μg/mL, Mouse CKAP4/p63 binds with an ED50 of 15-90 ng/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 500 μg/mL in PBS.
Bioactivity-ELISA 1
Bioactivity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse Dkk-1 N-Terminal Fragment is coated at 1 μg/mL, Mouse CKAP4/p63 binds with an ED50 of 15-90 ng/mL.

Data Sheet MSDS
Datasheet MSDS
For research or industrial raw materials, not for personal medical use!

Online Inquiry
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  • 1-631-559-9269
    1-516-512-3133
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