Product Overview
Recombinant Mouse Dkk-2 protein (Ser26-Ile259), with Leu35Pro substitution and a C-terminal 10-His tag was expressed in Spodoptera frugiperda, Sf 21 (baculovirus) cells.
Description
Enables co-receptor binding activity. Involved in negative regulation of Wnt signaling pathway. Predicted to be located in extracellular region. Predicted to be active in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; eye; and genitourinary system. Orthologous to human DKK2 (dickkopf WNT signaling pathway inhibitor 2).
Bio-activity
Measured by its ability to inhibit Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. Recombinant Mouse Wnt-3a induces a dose responsive increase in Wnt reporter activity in HEK293 cells. Recombinant Mouse Dkk-2 inhibits a constant dose of 100 ng/mL of Recombinant Mouse Wnt-3a Recombinant Mouse Wnt-3a. The ED50 for this effect is 100-600 ng/mL.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
