Recombinant Mouse Epo Protein (27-192aa), C-His-Tagged
Product Description
Cat
IMP-9518
Official Symbol
Epo
Product Overview
Recombinant mouse EPO, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Description
EPO, also known as erythropoietin, is a glycoprotein hormone in the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types.
Expression System
Baculovirus
Species
Mouse
Tag
C-His
Form
Liquid in Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Molecular Mass
19.8 kDa (176 aa)
Protein length
27-192
AA Sequence
<ADP>MAPPRLICDSRVLERYILEAKEAENVTMGCAEGPRLSENITVPDTKVNFYAWKRMEVEEQAIEVWQGLSLLSEAILQAQALLANSSQPPETLQLHIDKAISGLRSLTSLLRVLGAQKELMSPPDTTPPAPLRTLTVDTFCKLFRVYANFLRGKLKLYTGEVCRRGDR<HHHHHH>
Endotoxin
< 1 EU/μg of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Applications
SDS-PAGE, Bioactivity
Notes
Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
Storage
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration
0.25 mg/mL (determined by Absorbance at 280nm)
SDS-PAGE
SDS-PAGE

3 μg by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

Bioactivity-ELISA 1
Biological Activity

Mouse EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 2ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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