Recombinant Mouse FAP Protein (Leu26-Asp761)
Product Description
Cat
IMP-9932
Official Symbol
Fap
Product Overview
Recombinant Mouse FAP protein (Leu26-Asp761) without tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
Tag Free
Predicted N Terminal
Leu26&Val31
Form
Supplied as a 0.2 μm filtered solution in Tris, NaCl and Glycerol.
Molecular Mass
Predicted Molecular Mass: 85 kDa
SDS-PAGE: 78-90 kDa, reducing conditions
Protein length
Leu26-Asp761
Bio-activity
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions.
Endotoxin
<1.0 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
3 months, -20 to -70 centigrade under sterile conditions after opening.
Bioactivity-ELISA 1
Enzyme Activity

Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000 pmol/min/μg, as measured under the described conditions.

Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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