Recombinant Mouse FAP protein, N-His Tag

Product Description

Cat

IMP-1294

Official Symbol

FAP

Product Overview

Recombinant Mouse FAP protein(Leu 26 - Asp 761)(Accession # P97321)., fused to His Tag at the N-terminus, was expressed in HEK293.

Expression System

HEK293

Species

Mouse

Tag

N-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 87.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 26 - Asp 761

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse FAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Mouse FAP, His Tag was more than 90% and the molecular weight of this protein is around 180-190 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Mouse FAP, His Tag at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.1-3 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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