Recombinant Mouse FAP protein, N-His Tag

Product Description

Cat

IMP-1294

Official Symbol

FAP

Product Overview

Recombinant Mouse FAP protein(Leu 26 - Asp 761)(Accession # P97321)., fused to His Tag at the N-terminus, was expressed in HEK293.

Expression System

HEK293

Species

Mouse

Tag

N-His Tag

Form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.

Molecular Mass

This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 87.1 kDa. The protein migrates as 100-110 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Protein length

Leu 26 - Asp 761

Endotoxin

Less than 1.0 EU per μg by the LAL method.

Purity

>95% as determined by reduced SDS-PAGE.
>90% as determined by SEC-MALS.

Storage

This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.

SDS-PAGE

Mouse FAP, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS 1

The purity of Mouse FAP, His Tag was more than 90% and the molecular weight of this protein is around 180-190 kDa verified by SEC-MALS.

Bioactivity-ELISA 1

Immobilized Mouse FAP, His Tag at 1 μg/mL (100 μL/well) can bind Anti-FAP Antibody, Mouse IgG2a (FAP5) with a linear range of 0.1-3 ng/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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