Recombinant Mouse FASLG Protein (Pro132-Leu279), N-6×His-tagged
Product Description
Cat
IMP-9830
Official Symbol
Fasl
Product Overview
Recombinant Mouse Fas Ligand/TNFSF6 protein (Pro132-Leu279) with an N-terminal Met and 6×His tag was expressed in Mouse myeloma cell line.
Description
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
N-6×His
Predicted N Terminal
Met
Form
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Molecular Mass
Predicted Molecular Mass: 18 kDa (monomer)
SDS-PAGE: 27-33 kDa, reducing conditions
Protein length
Pro132-Leu279
Bio-activity
Measured by its ability to induce apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.25-1.5 μg/mL in the presence of 10 μg/mL of a cross-linking antibody Mouse Anti-polyHistidine Monoclonal Antibody. This soluble Recombinant Mouse Fas Ligand/TNFSF6 has weak cytotoxic activity. It has no effects on A20 mouse B cell lymphoma cells which express mouse Fas. This is one of multiple forms available for this protein. Check R&D Systems' website, www.RnDSystems.com, for a complete listing of the products.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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