Recombinant Mouse FCER2 Protein (Glu50-Pro331), N-6×His-tagged
Product Description
Cat
IMP-9850
Official Symbol
Fcer2a
Product Overview
Recombinant Mouse CD23/Fc epsilon RIIA protein (Glu50-Pro331) with an N-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
N-6×His
Predicted N Terminal
His
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 32.7 kDa
SDS-PAGE: 40-43 kDa, reducing conditions
Protein length
Glu50-Pro331
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant mouse CD23 is immobilized at 5 μg/mL (100 μL/well), it binds to mouse IgE in a range of 0.02-10 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
Data Sheet MSDS
For research or industrial raw materials, not for personal medical use!

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