Recombinant Mouse FCER2 Protein (Glu50-Pro331), N-6×His-tagged
Product Description
Cat
IMP-9850
Official Symbol
Fcer2a
Product Overview
Recombinant Mouse CD23/Fc epsilon RIIA protein (Glu50-Pro331) with an N-terminal 6×His tag was expressed in Mouse myeloma cell line.
Description
The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.
Expression System
Mouse myeloma cell line
Species
Mouse
Tag
N-6×His
Predicted N Terminal
His
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 32.7 kDa
SDS-PAGE: 40-43 kDa, reducing conditions
Protein length
Glu50-Pro331
Bio-activity
Measured by its binding ability in a functional ELISA. When recombinant mouse CD23 is immobilized at 5 μg/mL (100 μL/well), it binds to mouse IgE in a range of 0.02-10 μg/mL.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 250 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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