Recombinant Mouse Fcgr2b Protein, C-His&C-AVI-tagged

Product Description

Cat

IMP-4209

Official Symbol

Fcgr2b

Product Overview

Recombinant extracellular domain (Met 1-Arg 217) of mouse FGGR2B (NP_001070657.1) as fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Description

The protein encoded by this gene is a low affinity receptor for the Fc region of immunoglobulin gamma complexes. The encoded protein is involved in the phagocytosis of immune complexes and in the regulation of antibody production by B-cells. Variations in this gene may increase susceptibilty to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms have been found for this gene.

Expression System

HEK293

Species

Mouse

Tag

C-His&C-AVI

Predicted N Terminal

Thr 40

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The secreted recombinant mouse FGGR2B consists of 212 amino acids and has a predicted molecular mass of 23.9 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm FGGR2B is approximately 38-43 kDa due to glycosylation.

Protein length

Met1-Arg217

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 98 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Bioactivity-ELISA 1

Immobilized mouse CD32-AVI-His at 10 μg/mL (100 μL/well) can bind biotinylated Human IgG1, The EC50 of biotinylated Human IgG1 is 0.08-0.18 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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