Recombinant Mouse Fcgr4 Protein, C-His&C-AVI-tagged

Product Description

Cat

IMP-4483

Official Symbol

Fcgr4

Product Overview

The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.

Description

Enables IgE receptor activity and IgG receptor activity. Involved in neutrophil activation and positive regulation of bone resorption. Located in cell surface. Is expressed in liver. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); glomerulonephritis (multiple); hematologic cancer (multiple); and immunodeficiency 20. Orthologous to human FCGR3A (Fc fragment of IgG receptor IIIa) and FCGR3B (Fc fragment of IgG receptor IIIb).

Expression System

HEK293

Species

Mouse

Tag

C-His&C-AVI

Predicted N Terminal

Glu 19

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.

Protein length

Met1-Gln203

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 97 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-ELISA 1

Measured by its binding ability in a functional ELISA. Immobilized mouse CD16-2-AVI-His at 10 μg/mL (100 μL/well) can bind recombinant human IgG1 (Fc). The EC50 of human IgG1 (Fc) is 0.34-0.78 μg/mL.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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