Recombinant Mouse Fcgr4 Protein, C-His&C-AVI-tagged
Product Description
Cat
IMP-4483
Official Symbol
Fcgr4
Product Overview
The amino acid sequence corronding to (Met 1-Gln 203) of mouse FCGR4 (NP_653142.2) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus.
Description
Enables IgE receptor activity and IgG receptor activity. Involved in neutrophil activation and positive regulation of bone resorption. Located in cell surface. Is expressed in liver. Human ortholog(s) of this gene implicated in several diseases, including allergic disease (multiple); autoimmune disease (multiple); glomerulonephritis (multiple); hematologic cancer (multiple); and immunodeficiency 20. Orthologous to human FCGR3A (Fc fragment of IgG receptor IIIa) and FCGR3B (Fc fragment of IgG receptor IIIb).
Expression System
HEK293
Species
Mouse
Tag
C-His&C-AVI
Predicted N Terminal
Glu 19
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.
Protein length
Met1-Gln203
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 97 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-ELISA 1
Bioactivity-ELISA

Measured by its binding ability in a functional ELISA. Immobilized mouse CD16-2-AVI-His at 10 μg/mL (100 μL/well) can bind recombinant human IgG1 (Fc). The EC50 of human IgG1 (Fc) is 0.34-0.78 μg/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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