Recombinant Mouse Fcgrt&B2m Protein, C-His-tagged
Product Description
Cat
IMP-4493
Official Symbol
Fcgrt&B2m
Product Overview
Recombinant extracellular domain (Met 1-Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant mouse B2M (P01887, 105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified
Description
This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.
Expression System
HEK293
Species
Mouse
Tag
C-His
Predicted N Terminal
Ala 24&Ile 21
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant heterodimer of mouse FCGRT / B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of FCGRT / B2M heterodimer is approximately 35&12 kDa respectively in SDS-PAGE under reducing conditions
Protein length
Met1-Ser297 and Met1-Met119
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 95 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-BLI 1

Labeled biotin to FCGRT&B2M Heterodimer Protein, Mouse, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT&B2M Heterodimer Protein, Mouse, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 1.6 nM.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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