Recombinant Mouse Fcgrt&B2m Protein, C-His-tagged

Product Description

Cat

IMP-4493

Official Symbol

Fcgrt&B2m

Product Overview

Recombinant extracellular domain (Met 1-Ser 297) of mouse FCGRT (Q61559) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; Recombinant mouse B2M (P01887, 105Ala/Asp) (Met 1-Met 119) constructed the plasmid 2. The two plasmids were co-expressed and the FCGRT / B2M heterodimer was purified

Description

This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.

Expression System

HEK293

Species

Mouse

Tag

C-His

Predicted N Terminal

Ala 24&Ile 21

Form

Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.

Molecular Mass

The recombinant heterodimer of mouse FCGRT / B2M comprises 385 (285 + 100) amino acids and has a calculated molecular mass of 43.5 (31.8 + 11.7) kDa. The apparent molecular mass of FCGRT / B2M heterodimer is approximately 35&12 kDa respectively in SDS-PAGE under reducing conditions

Protein length

Met1-Ser297 and Met1-Met119

Endotoxin

< 1.0 EU/μg of the protein as determined by the LAL method

Purity

> 95 % as determined by SDS-PAGE

Storage

Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.

Reconstitution

A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

SDS-PAGE

Bioactivity-BLI 1

Labeled biotin to FCGRT&B2M Heterodimer Protein, Mouse, Recombinant (His Tag) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of FCGRT&B2M Heterodimer Protein, Mouse, Recombinant (His Tag), Biotinylated bound to IgG1 Antibody was 1.6 nM.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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