Recombinant Mouse Flt3l Protein, C-hFc-tagged
Product Description
Cat
IMP-4517
Official Symbol
Flt3l
Product Overview
Recombinant mouse FLT3L (P49772-1) (Met1-Arg188) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Description
FLT3L, also known as flt3 ligand, is a small molecule that acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. In vivo, FLT3L also induces the mobilization of the hematopoietic progenitors and stem cells. This may help the system to kill cancer cells. Dendritic cells (DCs) provide the key link between innate and adaptive immunity by recognizing pathogens and priming pathogen-specific immune responses. FLT3L controls the development of DCs and is particularly important for plasmacytoid DCs and CD8 -positive classical DCs and their CD103 -positive tissue counterparts.
Expression System
HEK293
Species
Mouse
Tag
C-hFc
Predicted N Terminal
Gly 27
Form
Lyophilized from sterile PBS, pH 7.4, 5 % trehalose, 5% mannitol and 0.01% Tween80.
Molecular Mass
The recombinant mouse FLT3L /Fc is a disulfide-linked homodimer. The reduced monomer comprises 403 amino acids and has a predicted molecular mass of 45.4 kDa. The apparent molecular mass of the protein is approximately 56 and 35 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Protein length
Met1-Arg188
Endotoxin
< 1.0 EU/μg of the protein as determined by the LAL method
Purity
> 90 % as determined by SDS-PAGE
Storage
Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
SDS-PAGE
SDS-PAGE

Bioactivity-Cell based assay 1
Bioactivity-Cell based assay

Measured in a cell proliferation assay using BaF3 mouse pro­B cells transfected with mouse Flt­3. The ED50 for this effect is typically 7-30 ng/mL.

Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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