Recombinant Mouse FOLR1 Protein (Met1-Ser232), C-6×His-tagged
Product Description
Cat
IMP-10136
Official Symbol
FOLR1
Product Overview
Recombinant Mouse FOLR1 protein (Met1-Ser232) with a C-terminal 6×His tag was expressed in Chinese Hamster Ovary cell line.
Description
The protein encoded by this gene is a member of the folate receptor family. Members of this gene family bind folic acid and its reduced derivatives, and transport 5-methyltetrahydrofolate into cells. This gene product is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. Mutations in this gene have been associated with neurodegeneration due to cerebral folate transport deficiency. Due to the presence of two promoters, multiple transcription start sites, and alternative splicing, multiple transcript variants encoding the same protein have been found for this gene.
Expression System
CHO
Species
Mouse
Tag
C-6×His
Predicted N Terminal
Arg23
Form
Lyophilized from a 0.2 μm filtered solution in PBS.
Molecular Mass
Predicted Molecular Mass: 25.3 kDa
SDS-PAGE: 29-36 kDa, reducing conditions
Protein length
Met1-Ser232
Bio-activity
Measured in a competitive binding assay.
When Folic Acid-Bovine Serum Albumin is immobilized at 5 μg/mL (100 μL/well), Recombinant Mouse (rm) FOLR1 inhibits 50% binding of biotinylated rmFOLR1 (7.9 nM) at the concentration range of 30-120 nM.
Endotoxin
<0.10 EU/μg of the protein by the LAL method.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution
Reconstitute at 100 μg/mL in PBS.
Data Sheet MSDS
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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