Recombinant Mouse Ggh Protein, C-Myc/DDK-tagged

Product Description

Cat

IMP-7592

Official Symbol

Ggh

Product Overview

Purified recombinant protein of Mouse gamma-glutamyl hydrolase (Ggh), with C-terminal Myc/DDK tag, expressed in HEK293T cells.

Description

Hydrolyzes the polyglutamate sidechains of pteroylpolyglutamates. Progressively removes gamma-glutamyl residues from pteroylpoly-gamma-glutamate to yield pteroyl-alpha-glutamate (folic acid) and free glutamate. May play an important role in the bioavailability of dietary pteroylpolyglutamates and in the metabolism of pteroylpolyglutamates and antifolates. Acts as endopeptidase. Lysosomal enzyme is activated by sulfhydryl compounds.[UniProtKB/Swiss-Prot Function]

Expression System

HEK293T

Species

Mouse

Tag

C-Myc/DDK

Form

25mM Tris.HCl, pH 7.3, 100mM glycine, 10% glycerol

Molecular Mass

35.4 kDa

AA Sequence

MANLGYLLCLLGLLLCGLSSPGMSRPYNHGSERPIIGVVMQECFGKMAKLGNYYIAASYVKYIESAGARVVPIRPDLSDAEYEELFRSINGVLLPGGGANLTDSGYSRVAKIFFSKALESFDNGGHFPVWGTCLGFEELSVLVSGENLLTSTDTKSKKLPLNFTEGARKSRMFKHFPTELLDSLALENLTANFHKWSLSVKNFTENEKLKKFFNILTTNTDGKTEFISSMEGFKYPVYAVQWHPEKAPFEWKNLGGISHAPNAVKTSFYLAEFLVSEARKNSHHFENVVKETASLIYKVYPIYTGNISSFQQAYMFDTRTRPLEQKLISEEDLAANDILDYKDDDDKV

Purity

> 80% as determined by SDS-PAGE and Coomassie blue staining.

Storage

Store at -80 centigrade after receiving vials.
Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.

Concentration

> 50 μg/mL as determined by microplate BCA method.

Data Sheet MSDS

Datasheet MSDS

For research or industrial raw materials, not for personal medical use!

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